| 產(chǎn)品名稱 |
HFBCC16 |
| 商品貨號(hào) |
B212778 |
| Designations |
HFBCC16 |
| GenBank Number |
M78440
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 5.599999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDC27*
Genomic copy number: unique
Gene name: cell division cycle 27 (human homolog of S. pombe nuc2+ and
A. nidulans bimA)
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q21-q24
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.6
Cross references: DNA Seq. Acc.: M78440; EST: EST00588; Primer: M13 Forward Insert lengths(kb): 2.599999904632568 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: cell division cycle 27 (human homolog of S. pombe nuc2+ and A. nidulans bimA) [CDC27*] |
| Insert Size (kb) |
2.600 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.6; PstI--3.0, 1.7, 0.8; HindIII--5.2, 0.3. According to the coding-region prediction program CRM, these sequence ranges (followed by the probability) of EST00588 may contain coding sequence: 101-151 (good). |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Tugendreich S, et al. Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag database. Proc. Natl. Acad. Sci. USA 90: 10031-10035, 1993. PubMed: 8234252
J C Venter, personal communication
|
