Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.2, 3.7, 2.7, 0.2; EcoRI--4.3, 3.2, 2.9, 0.3; SacI--8.0, 2.8. The insert contains the following restriction sites (according to genomic position): XhoI--1642; BamHI--1926, 2148, 7265; EcoRI--2414, 2474, 5646, 6105; BglI--3408. The clone is infectious. Contains the compete genome, interrupted at the SacI site that occurs at nt 5822. |