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pOM3
pOM3
規(guī)格:
貨期:
編號:B222507
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pOM3
商品貨號 B222507
Designations pOM3
Depositors I Oberbaumer
Biosafety Level 1
Vector Information
Size (kb): 2.7130000591278080
Vector: pOM3 (plasmid)
Promoters: Promoter lac
Construction: pUC9, M13tg131
Marker(s):ampR
Construct size (kb): 2.713000059127808
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
expression vector
vector containing primer sites useful for sequencing
vector for constructing cDNA libraries
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7.
Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation.
pOM3 (ATCC 77222) and pOM4 (ATCC 77223) differ in the orientation of the polylinker.
Contains an additional ATG in the inserted ClaI linker in a different reading frame which might also function as start codon for expression.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975

Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992.

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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