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OL11
OL11
規(guī)格:
貨期:
編號(hào):B224082
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) OL11
商品貨號(hào) B224082
Designations OL11
Species Homo sapiens, human
Depositors C Wappenschmidt
Applications
Only insert DNA should be used for nick translation.
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 0.5
Tissue: lymphocyte
Gene product: DNA Segment, single copy [D12S10]
Alleles: A1, A2
Insert Size (kb) 0.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.8, 0.84, 0.37; BamHI--4.1, 0.82; SalI/HindIII--3.9, 0.66, 0.39; BamHI/HindIII--4.3, 0.6; ClaI--4.9; EcoRI--5.2, 0.5.
MspI identifies 2 additional polymorphisms. Only insert DNA should be used for nick translation.
Codominant segregation demonstrated in 4 informative families with 22 individuals.
Enzyme(s) not detecting polymorphism: BamHI, BglII, HindIII, PvuII, XbaI with a panel of 9 unrelated Caucasians.
References

Wappenschmidt C, et al. Human cDNA probe (OL11) detects RFLP on chromosome 12 (D12S10). Nucleic Acids Res. 15: 2398, 1987. PubMed: 2882473

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