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EY2 [lambdaLASEY2]
EY2 [lambdaLASEY2]
規(guī)格:
貨期:
編號:B232465
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EY2 [lambdaLASEY2]
商品貨號 B232465
Designations EY2 [lambdaLASEY2]
Species Homo sapiens, human
Depositors A Swaroop
Origin
eye cell line
Vector
Tissue: eye, cell line CRL-7475
Type of insert: cDNA
Insert size range: > 0.8 kb
Genome Equivalent: 0.0
Genome: human
Vector : Charon BS-
Insert size range (kb) : > 0.8 kb
Vector ends : Modification : EcoRI HindIII
Number of independent recombinants : 1.0 x 106
Titer : 1.3 x 108 pfu/ml
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Frozen bacteria-free phage lysate.
Comments
cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
References

Swaroop A. Construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure. Methods Neurosci. 15: 285-300, 1993.

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

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