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PEmbl 600 [pEmbl 600 q]
PEmbl 600 [pEmbl 600 q]
規(guī)格:
貨期:
編號(hào):B235462
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 PEmbl 600 [pEmbl 600 q]
商品貨號(hào) B235462
Designations PEmbl 600 [pEmbl 600 q]
Species Homo sapiens, human
Applications
DNA from secondary transfectants was used to isolate the clone.
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 0.6000000238418579
Tissue: NK14 cell line DNA transferred into NIH 3T3 cells
Gene product: mel transforming oncogene (derived from cell line NK14)- RAB8 homologue(ras-associated protein RAB8) [MEL]
Insert Size (kb) 0.600
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--3.85, 0.69; ClaI--4.4; EcoRI--4.4; SalI--4.4; HindIII--4.4.
Contains Sau3A (225), AccI (300), HinfI (345), DdeI (365), and SacII (540) sites (approximate distances in bp from 5' end). Suggest using the AvaI/Sau3A fragment as there are 50 bp of Alu sequences 3' to Sau3A site.
NIH 3T3 cells were transformed with DNA from the melanoma NK14 cell line. DNA from secondary transfectants was used to isolate the clone.
References

Padua RA, et al. A novel transforming gene in a human malignant melanoma cell line. Nature 311: 671-673, 1984. PubMed: 6090953

Spurr NK, et al. Chromosomal assignment of c-mel, a human transforming oncogene, to chromosome 19 (p13.2-q13.2). Somatic Cell Mol. Genet. 12: 637-640, 1986. PubMed: 3466361

Rose. Ann Padua, personal communication

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