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pCT5-MK18 H27dD28:AA
pCT5-MK18 H27dD28:AA
規(guī)格:
貨期:
編號:B208561
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCT5-MK18 H27dD28:AA
商品貨號 B208561
Designations pCT5-MK18 H27dD28:AA
Species Mus musculus, mouse
Depositors S Paul
Applications
produces protein immunoglobulin light chain, anti-VIP
Vector
Construct size (kb): 5.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 0.800000011920929
Tissue: hyperimmunized spleen, B-lymphocytes
Gene product: immunoglobulin light chain
Target Gene: immunoglobulin light chain, anti-VIP
Insert Size (kb) 0.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): NotI/SfiI--5.3; BstEII--5.3; EcoRI/HindIII--3.2, 2.1; BamHI--5.3.
Insert was isolated from hybridoma cell line clone c23.5 producing anti-VIP (vasoactive intestinal peptide) monoclonal antibodies.
Insert encodes the following mutant form: His27d --> Ala, Asp28 --> Ala.
The following nucleotides differ from the published sequence, but do not alter the amino acid sequence: nt 3 T->C; nt 6 G->T; nt 9 G->A.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Gao QS, et al. Molecular cloning of a proteolytic antibody light chain. J. Biol. Chem. 269: 32389-32393, 1994. PubMed: 7798238

Gao QS, et al. Site-directed mutagenesis of proteolytic antibody light chain. J. Mol. Biol. 253: 658-664, 1995. PubMed: 7473741

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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