產(chǎn)品名稱 | WT 9-7 |
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商品貨號 | B208743 |
Organism | Homo sapiens, human |
Tissue | kidney, cortex, proximal tubule, cyst |
Cell Type | epithelial |
Product Format | frozen |
Morphology | polygonal |
Culture Properties | adherent |
Biosafety Level | 2 [Cells contain SV-40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | autosomal dominant polycystic kidney disease (ADPKD) |
Age | unknown |
Gender | female |
Applications | These new series of cyst-derived cell lines represent useful in vitro models for studying the cellular and molecular biology of ADPKD. |
Storage Conditions | liquid nitrogen vapor phase |
Derivation | The WT 9-7 cell line was established in July, 1999 from a single cyst from a proximal cortical tubule taken from a patient with Autosomal dominant polycystic kidney disease (ADPKD). WT 9-7 is one of a series of immortalized epithelial cell lines from over 30 individual renal cysts obtained from 11 patients with ADPKD. The cells were transformed with a wild type recombinant adeno-SV40 virus. |
Antigen Expression | alanyl (membrane) aminopeptidase (ANPEP, CD13); Homo sapiens, expressed Ref![]() ![]() ![]() ![]() large T antigen; Simian virus 40, expressed Ref ![]() ![]() |
Receptor Expression | epidermal growth factor (EGF), expressed Ref![]() ![]() |
Genes Expressed | angiotensinogen Ref
![]() ![]() polycystic kidney disease 1 Ref
![]() ![]() polycystic kidney disease 2 Ref
![]() ![]() Ref
![]() ![]() |
Cellular Products | angiotensinogen Ref
![]() ![]() polycystic kidney disease 1 Ref
![]() ![]() polycystic kidney disease 2 Ref ![]() ![]() |
Comments | These viruses contain an origin-defective (ori-) WT SV40 DNA cloned into the adenovirus vector in place of regions 1a and 1b. Ref![]() ![]() |
Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Note: Flasks must be coated with Bovine Collagen type I solution (supplied stock concentration is 3.0 mg/mL; PureCol® Advanced Biomatrix Catalog No. 5005-B) for 1 hour at 37°C
Interval: Maintain cultures at a cell concentration between 5 x 103 and 5 x 104 cells/cm2
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: Two to three times weekly
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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Culture Conditions | Temperature: 37°C |
Population Doubling Time | 26 hours |
Name of Depositor | M Loghman-Adham |
Year of Origin | July, 1999 |
References | Loghman-Adham M, et al. Immortalized epithelial cells from human autosomal dominant polycystic kidney cysts. Am. J. Physiol. Renal Physiol. 285: F397-F412, 2003. PubMed: 12734101 Loghman-Adham M, et al. An intrarenal renin-angiotensin system in autosomal dominant polycystic kidney disease. Am. J. Physiol. Renal Physiol. 287: F775-F788, 2004. PubMed: 15187005 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
梅經(jīng)理 | 17280875617 | 1438578920 |
胡經(jīng)理 | 13345964880 | 2438244627 |
周經(jīng)理 | 17757487661 | 1296385441 |
于經(jīng)理 | 18067160830 | 2088210172 |
沈經(jīng)理 | 19548299266 | 2662369050 |
李經(jīng)理 | 13626845108 | 972239479 |