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HHCMH42
HHCMH42
規(guī)格:
貨期:
編號(hào):B208750
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HHCMH42
商品貨號(hào) B208750
Designations HHCMH42
GenBank Number

M78047

Species Homo sapiens, human
Vector
Construct size (kb): 4.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S1050E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: TAX
Other gene symbols: TAG1
Genomic copy number: unique
Gene name: transiently-expressed axonal glycoprotein (axonin-1)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 q32
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.9
Cross references: DNA Seq. Acc.: M78047; EST: EST01634; Primer: M13 Reverse;
THC id: THC109655
DNA Seq. Acc.: X68274
Nucleotides 1-237 of the insert correspond to
nucleotides 2690-2925 of X68274 with 97% identity.
Insert lengths(kb): 1.899999976158142
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)
Gene product: axonal glycoprotein TAG-1( axonal glycoprotein TAG-1, axonin-1 cell adhesion molecule, transiently-expressed axonal glycoprotein, transiently-expressed axonal glycoprotein (axonin-1)) [TAX]
Insert Size (kb) 1.900
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.9; PstI--3.1, 1.1, 0.34, 0.21; BamHI--4.2, 0.50.
EST01634 matches PIR accession A34695 with 87.14% identity and 94.28% similarity over the sequence range: 1-210.
Sequenced from the M13 reverse primer.
This sequence record is for rat axonal glycoprotein TAG-1.
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Hasler TH, et al. cDNA cloning, structural features, and eukaryotic expression of human TAG-1/axonin-1. Eur. J. Biochem. 211: 329-339, 1993. PubMed: 8425542

J C Venter, personal communication

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