| 產(chǎn)品名稱 | BAX KO SV40 MEF |
|---|---|
| 商品貨號 | B208968 |
| Organism | Mus musculus, mouse |
| Tissue | embryonic fibroblast |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast-like |
| Culture Properties | adherent |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, BAX function, as well as cells that are resistant to multiple apoptotic stimuli. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Cells are immortalized MEFs, which were generated from BAX knockout mice. |
| Tumorigenic | no |
| Comments | Cells are immortalized MEFs (genotype: bax-/-), which were generated from BAX knockout mice. BAX is a pro-apoptotic BCL-2 family member. Cells are sensitive to tBID-induced cytochrome c release and apoptosis.
Cells are sensitive to multiple apoptotic stimuli that act through disruption of mitochondrial function: staurosporine, ultraviolet radiation, growth factor deprivation, etoposide, and the endoplasmic reticulum stress stimuli thapsigargin and tunicamycin.
Cells are sensitive to Bim and Bad expression induced apoptosis. This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, BAX function, as well as cells that are resistant to multiple apoptotic stimuli.
|
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated IMDM Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 10 % Fetal Bovine Serum and 1x Non-essential amino acids.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:8 to 1:30 is recommended. Medium renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: 90% Fetal Bovine Serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor | S Korsmeyer |
| References | Wei M, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 292(5517): 727-730, 2001 PubMed: 11326099 Lindsten T, et al. The Combined Functions of Proapoptotic Bcl-2 Family Members Bak and Bax Are Essential for Normal Development of Multiple Tissues. Molecular Cell 6(6): 1389-1399, 2000 PubMed: 11163212 Zong WX, et al. BH3-only proteins that bind pro-survival Bcl-2 family members fail to induce apoptosis in the absence of Bax and Bak. Genes Dev. 15(12): 1481-6, 2001 PubMed: 11410528 Cheng EH, et al. BCL-2, BCL-XL Sequester BH3 Domain-Only Molecules Preventing BAX- and BAK-Mediated Mitochondrial Apoptosis. Molecular Cell 8(3) : 705–711, 2001 PubMed: 11583631 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
