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Human rhinovirus 39
Human rhinovirus 39
規(guī)格:
貨期:
編號:B209430
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human rhinovirus 39
商品貨號 B209430
Classification Picornaviridae, Enterovirus, Rhinovirus A
Deposited As Rhinovirus 39
Agent Human rhinovirus 39
Strain 209
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Growth not inhibited by IUDR. Stabilized by 1 M MgCl2. Does not hemagglutinate rat, rhesus monkey, or human blood type O. Non-pathogenic for suckling mice.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
H1-HeLa (ATCC® CRL-1958™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 1-7 days while rocking continuously
Name of Depositor MA Mufson, National Institute of Allergy and Infectious Diseases; RM Conant, VV Hamparian, The Ohio State University
Source Nasal washing from an adult female, North Carolina
References

Mufson MA, et al. A description of six new Rhinoviruses of human origin. Am. J. Epidemiol. 81: 32-43, 1965. PubMed: 14246079

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