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HFBCN94
HFBCN94
規(guī)格:
貨期:
編號:B209449
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HFBCN94
商品貨號 B209449
Designations HFBCN94
GenBank Number

M85806

Species Homo sapiens, human
Applications
The predicted product size is (bp): 112. The observed product size(s) is(are) (bp): 113 (human).
Vector
Construct size (kb): 5.25
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D5S1557E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 5; Localization: 5 q31.3-q33.2
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.25
Cross references: DNA Seq. Acc.: M85806; EST: EST02327; Primer: M13 Reverse
Insert lengths(kb): 2.25
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: DNA Segment, single copy, expressed [D5S1557E]
Insert Size (kb) 2.25
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.25; PstI--3.8, 1.0, 0.5; HindIII--5.3.
EST02327 can be detected in genomic DNA using the following oligonucleotides (5'-3'): CCCAGTAAACCCATCAGACT and ACATGTTTTGGGAGGGAC.
EST02327 matches GenBank accession HUMBFXIII (GenBank M64554, human factor XIII b) with 83.06% identity over the sequence range: 61-125.
EST02327 contains an O family repetitive element.
Sequenced from the M13 reverse primer.
The predicted product size is (bp): 112. The observed product size(s) is(are) (bp): 113 (human).
Related product(s): oligonucleotides, ATCC 73231.
References

Feldblyum TV, et al. Regional assignment and tissue expression of twenty-three expressed sequence tags (ESTs) from human chromosome 5. Genomics 33: 128-130, 1996. PubMed: 8617497

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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