Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; HindIII--5.4; PstI--4.1, 0.9.
Insert covers about 40% of the mRNA sequence (3' end) including coding for domain 2 and all of domain 3 of the protein.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
References
Morinaga T, et al. Primary structures of human alpha-fetoprotein and its mRNA. Proc. Natl. Acad. Sci. USA 80: 4604-4608, 1983. PubMed: 6192439
Nakao K, et al. c-Ha-ras down regulates the alpha-fetoprotein gene but not the albumin gene in human hepatoma cells. Mol. Cell. Biol. 10: 1461-1469, 1990. PubMed: 1690841
Murray JC, et al. Linkage disequilibrium of ALB and AFP RFLPs predicts 5' to 3' orientation. Cytogenet. Cell Genet. 40: 708, 1985.
Morinaga T, et al. Afphafetoprotein messenger RNA in human embryonal carcinoma grown in nude mice, and cloning of its complementary DNA. Oncodev. Biol. Med. 3: 301-313, 1982. PubMed: 6182554
Murray JC, et al. RFLPs for the human alphafetoprotein (AFP), at 4q11-4q13. Nucleic Acids Res. 13: 6794, 1985. PubMed: 2414731
