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YCp7-32phocat
YCp7-32phocat
規(guī)格:
貨期:
編號(hào):B209840
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 YCp7-32phocat
商品貨號(hào) B209840
Designations YCp7-32phocat
Depositors H Feldmann
Biosafety Level 1
Vector Information
Size (kb): 8.0600004196167000
Vector: YCp7-32phocat (plasmid)
Promoters: Promoter PHO5
Construction: YRp7-32phocat (ATCC 37625), CEN6
Marker(s):TRP1,ampR
Construct size (kb): 8.0600004196167
Features: marker(s): ampR, TRP1
promoter: PHO5
replicon: pMB1, ARS1
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 1.55; PstI--6.7, 1.35; PvuII--6.2, 2.05.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 1520 bp NdeI fragment containing CEN6 was cloned into the NdeI site of YRp7-32phocat (ATCC 37625).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

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