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Construct size (kb): 4.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: AQP4
Gene name: aquaporin 4
Contains complete coding sequence?: N
Tissue: brain
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.6
Cross references: DNA Seq. Acc.: U14007
Nucleotides 1-1588 of the insert correspond to
nucleotides 7-1595 of U14007.

Insert lengths(kb): 1.600000023841858

Tissue: brain

Gene product: aquaporin 4 [AQP4]

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.6; HindIII--4.2, 0.3; SmaI--3.7, 0.85; KpnI--4.6; SstI--3.8, 0.8.

Encodes a mercury-resistant water channel expressed primarily in rat adult brain.