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hPKCalpha-GF29
hPKCalpha-GF29
規(guī)格:
貨期:
編號:B210421
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 hPKCalpha-GF29
商品貨號 B210421
Designations hPKCalpha-GF29
GenBank Number

X52479

Species Homo sapiens, human
Depositors H Hug
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.244999885559082
Tissue: acute T cell leukemia, Jurkat cell line
Gene product: protein kinase C, alpha [PKCA]
Insert Size (kb) 2.245
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.3; BamHI--4.5, 0.65, 0.25; BglI--2.7, 1.3 (doublet); NcoI--4.2, 1.2; PvuII--2.4, 1.4, 0.75, 0.6.
The insert contains the following restriction sites (bp from the 5' end): BamHI--585, 796; BglI--965; NcoI--26, 1178; PstI--178; PvuII--1233, 1951; SmaI--44.
Contains the complete coding sequence (nt 28-2046).
The insert is oriented such that transcription from T7 gives the sense strand and from T3 gives the antisense strand.
References

Finkenzeller G, et al. Sequence of human protein kinase C alpha. Nucleic Acids Res. 18: 2183, 1990. PubMed: 2336401

Hubert Hug, personal communication

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