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produces protein estrogen receptor

Construct size (kb): 4.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pIC20H
Intact vector size: 2.722
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI
EcoRV BglII XhoI NruI
Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI ClaI
EcoRV XbaI BglII XhoI SacI NruI HindIII
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L08912

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ESR
Genomic copy number: unique
Gene name: estrogen receptor
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 q25.1
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: M12674

Insert lengths(kb): 1.799999952316284

Gene product: estrogen receptor [ESR]

Alleles: A1, A2

Target Gene: estrogen receptor

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.8; BglI--2.0, 1.6, 1.15; BglII--4.1, 0.54; HindIII--2.8, 1.0, 0.9; PstI--3.3, 1.0, 0.4, 0.2; NotI--4.6; BamHI--4.5.

The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.47; BglII--1.27; HindIII--1.02; NcoI--1.18; NotI--0.19; PstI--0.36, 1.32, 1.49; PvuII--0.35; SmaI--0.24; StuI--0.62, 0.71, 1.48; XbaI--1.13.

Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies.

It binds estrogens, antiestrogens, and DNA.

The recombinant fusion protein contains an additional 23 N-terminal amino acids encoded by the vector polylinker.